GDOI ENDONUCLEASE Meaning and
Definition
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GdoI endonuclease is a type II restriction enzyme that recognizes a specific DNA sequence and cleaves it at a specific site within that sequence. It belongs to the PD-(D/E)XK superfamily of nucleases, which are known for their ability to cleave DNA phosphodiester bonds.
GdoI endonuclease is derived from the bacteria Geobacillus stearothermophilus and is widely used in molecular biology research for DNA manipulation. This enzyme is a homodimer, meaning it consists of two identical subunits, and each subunit is responsible for the cleavage activity.
The recognition sequence for GdoI endonuclease is 5'...CTCGAG...3', where the caret indicates the cleavage site. When GdoI encounters this sequence, it binds to it with high specificity, and upon binding, it introduces a double-stranded DNA break by cleaving the DNA backbone just upstream of the central G residue on each strand. The resulting DNA fragments have a 4-nucleotide overhang with a 5' phosphate on one strand and a 3' hydroxyl on the other.
Because GdoI endonuclease recognizes a palindromic DNA sequence, it can create cohesive or sticky ends that are complementary to each other. This property makes it particularly useful in DNA cloning and molecular biology techniques such as restriction digestion, ligation, and gene insertion.
Overall, GdoI endonuclease is a valuable tool in molecular biology research due to its sequence specificity, enzyme activity, and ability to generate compatible ends for DNA manipulation.
Common Misspellings for GDOI ENDONUCLEASE
- fdoi endonuclease
- vdoi endonuclease
- bdoi endonuclease
- hdoi endonuclease
- ydoi endonuclease
- tdoi endonuclease
- gsoi endonuclease
- gxoi endonuclease
- gcoi endonuclease
- gfoi endonuclease
- groi endonuclease
- geoi endonuclease
- gdii endonuclease
- gdki endonuclease
- gdli endonuclease
- gdpi endonuclease
- gd0i endonuclease
- gd9i endonuclease
- gdou endonuclease
- gdoj endonuclease
Etymology of GDOI ENDONUCLEASE
The word "GdoI endonuclease" does not have an extensive etymology as it is a scientific term that refers to a specific type of enzyme. However, we can break down the word into its components:
1. "GdoI" refers to a specific restriction enzyme that recognizes a particular DNA sequence and functions as an endonuclease. It is named after the bacteria from which it was originally isolated or characterized.
2. "Endonuclease" is a compound word combining "endo" meaning "within" and "nuclease" meaning "a type of enzyme that breaks down nucleic acids (DNA or RNA)". Therefore, an endonuclease is an enzyme that cuts or cleaves DNA or RNA at specific internal sites.
Similar spelling word for GDOI ENDONUCLEASE
