POLYBRENE Meaning and
Definition
-
Polybrene, also known as hexadimethrine bromide, is a cationic polymer commonly used in molecular biology and cell culture applications. It is a highly positively charged compound due to its quaternary ammonium groups, which make it useful for enhancing transfection efficiency and promoting viral infection.
Polybrene is primarily employed as a transfection reagent to increase the uptake and expression of nucleic acids in cells, particularly in genetic engineering experiments. It facilitates the transfer of genetic material, such as DNA or RNA, into target cells by neutralizing the negative charge of the cell membrane and forming a complex with the negatively charged nucleic acids. This complex allows for improved electrostatic binding and subsequent internalization of nucleic acids by the cells.
Additionally, Polybrene is also utilized in virology experiments as a medium for enhancing viral infection capabilities. It can enhance viral attachment to target cells by neutralizing the negative charges on the cell surface and aiding in the binding of viral particles. This property makes it a valuable tool for optimizing viral transductions or generating viral particles for cell-based assays.
However, it is essential to note that Polybrene may show cytotoxic effects at higher concentrations. Careful optimization and titration are required to determine the suitable working concentration based on cell type and experimental requirements. Overall, Polybrene plays a critical role in molecular biology and cell culture, enabling efficient nucleic acid transfer and viral infection in various cellular systems.
Common Misspellings for POLYBRENE
- oolybrene
- lolybrene
- -olybrene
- 0olybrene
- pilybrene
- pklybrene
- pllybrene
- pplybrene
- p0lybrene
- p9lybrene
- pokybrene
- popybrene
- pooybrene
- poltbrene
- polgbrene
- polhbrene
- polubrene
- pol7brene
- pol6brene
- polyvrene
Etymology of POLYBRENE
The word "Polybrene" is derived from its chemical structure and composition. "Poly" refers to the presence of multiple elements or components in the compound, and "brene" is a combination of "bromine" and "ethylene". Polybrene is a cationic polymer composed of multiple ethylene bridges with bromine groups attached, giving it its name.
Infographic
Add the infographic to your website:
